Acid-bath Phosphorylation in Heptane-extracted Chloroplasts
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چکیده
منابع مشابه
Acid-bath Phosphorylation in Heptane-extracted Chloroplasts.
When spinach (Spinacia oleracea) chloroplast fragments were lyophilized and extracted with n-heptane, acid-bath phosphorylation was significantly reduced. Phosphorylation could be restored to these chloroplasts, if the extracted material (containing quinones, carotenoids, and other lipids) was added back to dry preparation before rehydration. The results reported suggest it was not quinones whi...
متن کاملA Lipid Requirement for Photosystem I Activity in Heptane-extracted Spinach Chloroplasts.
A lipid requirement for photosystem I activity in Spinacia oleracea chloroplasts has been characterized. The transfer of electrons from tetramethyl-p-phenylenediamine through the chloroplast photosystem to viologen dye was used as an assay of photosystem I activity. Activity is diminished by prolonged heptane extraction and is partially restored by readdition of the extracted lipid. Extracted c...
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In an earlier note, evidence was presented for the occurrence of a new type of chloroplast-catalyzed photosynthetic phosphorylation called “oxidative photophosphorylation” (I). When spinach chloroplasts are illuminated in the presence of orthophosphate, adenosine diphosphate, and a dye such as trichlorophenol indophenol, adenosine triphosphate is generated provided oxygen is available, but not ...
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In spinach chloroplasts which have been shaken with n-heptane, photophosphorylation coupled to the reduction of nicotinamide adenine dinucleotide phosphate is inhibited about 95% while the photoreduction of NADP+ (and ferricyanide) is stimulated as much as 50%. Photophosphorylation catalyzed by phenazine methosulfate, however, is inhibited only 50%. These data are interpreted to indicate that t...
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During attempts to prepare active fragments of spinach chloroplasts, we observed major losses of phosphorylative activity following suspension of the chloroplasts in water. This damage was traced to treatments which probably had the effect of removing cations from the chloroplasts, rather than to a simple physical disruption. Indeed, chloroplast fragments have been used successfully in studies ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1972
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.50.5.591